In vivo FRET imaging revealed a regulatory role of RanGTP in kinetochore-microtubule attachments via Aurora B kinase.

In vivo FRET imaging revealed a regulatory role of RanGTP in kinetochore-microtubule attachments via Aurora B kinase.

Blog Article

Under the fluctuating circumstances provided by the innate dynamics of microtubules and opposing tensions resulted from microtubule-associated motors, it is vital to ensure stable kinetochore-microtubule attachments for accurate segregation.However, a comprehensive understanding of how this regulation is mechanistically achieved remains elusive.Using our newly designed live cell FRET time-lapse imaging, we found that post-metaphase RanGTP is crucial in the Urinals maintenance of stable kinetochore-microtubule attachments by regulating Aurora B kinase via the NES-bearing Mst1.More importantly, our study demonstrates that by ensuring stable alignment of metaphase chromosomes prior to segregation, RanGTP is indispensible in governing the genomic integrity and the fidelity of E-Prom Chip cell cycle progression.

Our findings suggest an additional role of RanGTP beyond its known function in mitotic spindle assembly during the prometaphase-metaphase transition.